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Assessment of lysine released from rumen protected lysine products exposed to high and low moisture TMR over 24 hours.
Characteristics of a rumen protected lysine product. 2: Handling properties of the third-generation AjiPro®-L in feeding practices.
Characteristics of a rumen-protected lysine product. 1: Bioavailability of the third-generation AjiPro®-L.
Comparative bioavailability of lysine in three commercial rumen-protected lysine products using the in vivo plasma lysine response method.
Determination of the bioavailability of lysine in the latest generation of a rumen-protected lysine product exposed to TMR using the in vivo plasma lysine response method.
Determination of the comparative bioavailability of lysine in two rumen protected lysine products using the in vivo plasma lysine response method.
Determining the bioavailability of lysine in AjiPro®-L using the plasma-free amino acid dose response method.
It is quite important to evaluate the bio-availability of rumen protected amino acid products (RPAA) properly amid amino acid supply optimiztion has been getting more general in dairy diet formulation. The purpose of this trial was to establish an evaluation method for RPAA bio-availability assessment by making the best use of the nature of plasma amino acid concentrate increase corresponding proportionally to the dosage. By comparing the plasma lysine conc. increase along with the lysine incremental dosage out of abomasal infusion and AjiPro®-L feeding, the bio-availability of AjiPro®-L was estimated as 37% comparable to the spec value of AjiPro®-L.
Effect of rumen protected lysine and methionine on milk yield and composition in early lactation cows.
A trial to look into the effect of rumen-bypass lysine and methionine on milk yield and composition in the first 10 wks of lactation. Two diets were formulated, one has less lysine and methionine supply to the requirements and another has more of them with rumen protected lysine and methionine. Feed intake, FCM, milk protein percentage and yield were significantly improved when cows were fed high lysine and methionine diet in the early lactation.
Effect of rumen protected lysine and methionine on nitrogen utilization in early lactation cows.
A trial to study the effect of amino acid balancing through rumen protected lysine and methionine feeding on nitrogen utilization. Though negative control (less lysine and methionin to the requirements) made the FCM yeild, DM digestiblity and nitriongen utilization worse than those of positive control (lysine and methionine over the requirements without RPAA), RPAA supplementation tended to get them betterh and closer to those of positive control.
Effect of rumen protected lysine and methionine on productivity of early lactation dairy cows.
A trial to see the effect of all essential amino acids satisfied with the requirements on the latating performnace of early lactation multiparous cows. 36 dariry cows were divided into two groups and one group was fed lysine and methionine deficinet diet from the close-up through 6 weeks post-partum and another group was fed all amino acids sufficient diet with rumen protected lysine and methionine over the same period. Both group were fed the typical diet since 6 weeks after calving. The cows fed amino acid fortified diet could significantly increase the yield of milk, milk protein and lactose during the 6 weeks after parturition.
Effect of ruminally protected lysine and methionine on microbial yield, amino acid profile in the intestine, milk production and composition.
6 lactating cows equiped with ruminal and duodenal cannulas were used to determine the effect of rumen protected lysine and methonine on microbial yield, amino acid flow to duodenum, and lactating performance in a 3x3 Latin square arrangement of treatments. Milk protein, microbial synthesis in the rumen, and total EAA flow to duodenum were significantlv improved by supplementation of RP lysine & methionine.
Effects of a rumen protected lysine (AjiPro®-L) supplementation on peripartum disease, reproduction, and lactational performance of dairy cows.
Effects of feeding a ruminally protected lysine (AjiPro®-L) from calving to the fourth week of lactation on production of high-producing lactation dairy cattle.
A trial to look into the effect of AjiPro®-L feeding in fresh period on lactating performance of high-producing cows before and after treatment. 69 cows were equally allocated to 3 groups and fed one of three diets, basal diet, 100g or 200g AjiPro®-L supplemented diets for 4 weeks after parturitoin. The cows fed 200g rumen protected lysine could significantly increase milk yield, milk protein & fat yield and milk efficiency. 100g AjiPro®-L feeding also improved all of them but milk efficiency. Carry over effect was observed only in milk fat yield in both 100g and 200g AjiPro®-L treatments.
Effects of lysine and/or methionine oversupply on performance of lactating dairy cows.
Methionine, Lysine, both or none of them were infused into abomasum to multiparous dairy cows in mid lactation, fed a ration designed to be co-limiting in metabolizable methionine and lysine, in a 4 x 4 Latin square arrangement of treatments with 28 d periods. The infusion of either amino acid actually increased the intestinal delivery to between 150 and 160% of requirement. The overdose of either or both of amino acids depressed the lactating performance and it was in line with expectations based upon results of many studies with monogastric species showing that imbalanced profiles of digestible amino acids are associated with reduced intake and animal performance.
Evaluation of a ruminally protected lysine product to increase milk protein production and plasma lysine concentration.
Multiparous cows were used in a replicated 3 x 3 Latin square design to evaluate the efficacy of a rumin protected lysine product to increase milk protein production and plasma Lys concentration with 21 d periods. Cows were fed 1) the diet adequate in lysine, 2) the diet deficient in lysine or 3) the lysine deficient diet with rumen protected lysine to be adequate. There was no effect of the diet on any lactating performnace, but there was a significant effect on plasma Lys concentration with the Lys adequate diet resulting in the highest, the RPL diet was intermediate, and the Lys deficient diet was the lowest.
Feeding blood meal or two rumen protected lysine sources in early lactation dairy cows and the effect of withdrawal on production parameters.
Influence of supplementing high fiber diets with abomasally infused methionine and lysine, or isoleucine, or all three amino acids.
Isoleucine has been suggested to be a limiting amino aicd under some conditions. Methionine, Lysine, Isoleucin, all or none of them were ingested in rumen protected form or infused into abomasum to multiparous dairy cows fed a basal ration, designed to be co-limiting in intestinally absorbable supplies of methionine, lysine and isoleucine, in a 4 x 4 Latin square arrangement of treatments with 28 day periods. Milk protein % tended (P=0.07) to be higher when cows were supplemented with RP-lysine and -methionine. However production of milk and its components were not affected. Cows tended (P=O.O9) to have a higher milk lactose %, and tended (P=0.08) to produce more milk and(P=0.06) to produce more milk lactose when abomasally infused with isoleucine alone. However when cows were supplemented with all three AA, milk yield and composition did not differ from the control diet.
Productive responses of lactating dairy cattle to supplementing high levels of ruminally protected lysine using a rumen protection technology.
Rumen protected amino acid supplementation to dairy cows pre- and post-parturition
A trial to go over the effect of feeding rumen protected lysine and methionine in pre- and post- parturition on lactating performance and the interaction of the amino acid effect with the dietary CP level. Feeding rumen protected lysine and methionine over the period tended to increase milk and milk protein yield, and the effect was greater when lysine and methionine were fortified to high CP diet compared to low CP diet.
Ruminally protected lysine or lysine & methionine for lactating dairy cows fed to meet requirements for microbial and postruminal protein.
This study was designed to go over the effects of ruminally protected lysine separately from those of RP methionine on performance of lactating dairy cows fed a diet of which 1st limiting amino acid is lys and 2nd limiting is met. RP lysine, RP lysine & methionine or none of them were added to the basal diet at 5 weeks after parturition and the diets were fed for 20 weeks. RP lysince and/or methionine supplementation could not improve any performance since it was turned out that the diets have been deficient in other amino acids than lysine and methionine even with RP lysine and/or methinone feeding by the post-experimental calculation of the basal diet.
The effect of feeding ruminally protected lysine on production performance and plasma amino acid profile of early lactation dairy cattle.
A trial to examine the effects of rumen protected lysine supplementation and dosage on production performance and plasma amino acid profile of high-producing multiparous dairy cows. Four treatments were designed to deliver 0, 12, 24 and 36 g/cow/d more of intestinally available lysine with 75, 150, 225 g/cow/d RP lysine supplementation, respectively. Cows started the experimental period on the fifth week post-calving and remained on treatment for 4 weeks. Milk and protein yield were highest at 150 g/cow/d treatment, while FCM was highest at 75 g/cow/d treatment.